JVI：流感病毒具有调控细胞周期的作用
9月26日，国际知名学术杂志Journal of Virology在线发表了关于流感病毒调控宿主细胞周期的研究论文。这项研究成果由中科院上海巴斯德研究所孙兵研究员课题组完成。
在本研究中，研究人员观察了流感病毒对细胞周期的影响。研究结果表明，流感病毒A/WSN/33 (H1N1)的复制可以使细胞周期阻滞在G0/G1期。一些调控细胞周期由G0/G1期向S期转换的蛋白如高度磷酸化的Rb， p21，cyclinE，cyclinD1等在病毒感染后明显下调。更为重要的是，研究人员还发现流感病毒通过将细胞周期阻滞在G0/G1期，从而对病毒蛋白表达和病毒大量产生有重要作用。研究还发现一些其它流感病毒株也具有类似的作用。由此可见，这种作用有可能是流感病毒干预宿主细胞周期的一种普遍性机制。该发现为流感病毒致病机理和抗病毒药物的研究提供了新的思路。
该研究得到了国家自然科学基金委、中科院、上海市科委、国家863重点项目及李嘉诚基金会等项目资助。
推荐英文摘要：
Journal of Virology doi:10.1128/JVI.01316-10
Dendritic Cell Activation by Recombinant Hemagglutinin Proteins of H1N1 and H5N1 Influenza A Viruses
Wen-Chun Liu,1 Shih-Chang Lin,1 Yen-Ling Yu,2 Ching-Liang Chu,2* and Suh-Chin Wu1,2*
Institute of Biotechnology, National Tsing Hua University, Hsinchu 30013, Taiwan,1 Vaccine Research and Development Center, National Health Research Institutes, Zhunan, Miaoli County 350, Taiwan2
Since dendritic cells may play a key role in defense against influenza virus infection, we examined the effects of recombinant hemagglutinin (HA) proteins derived from mouse-adapted H1N1 (A/WSN/1933), swine-origin 2009 pandemic H1N1 (A/Texas/05/2009), and highly pathogenic avian influenza H5N1 (A/Thailand/KAN-1/2004) viruses on mouse myeloid dendritic cells (mDCs). The results reveal that tumor necrosis factor alpha (TNF-), interleukin-12 (IL-12) p70, and major histocompatibility complex class II (MHC-II) expression was increased in mDCs after treatment with recombinant HA proteins of H1N1 and H5N1. The specificity of recombinant HA treatments for mDC activation was diminished after proteinase K digestion. HA apparently promotes mDC maturation by enhancing CD40 and CD86 expression and suppressing endocytosis. No significant differences in mDC activation were observed among recombinant proteins of H1N1 and H5N1. The stimulation of mDCs by HA proteins of H1N1 and H5N1 was completely MyD88 dependent. These findings may provide useful information for the development of more-effective influenza vaccines.
